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Please read through the laboratory exercise and follow the directions. It is often helpful to look over the entire lab first before you begin. When you complete your laboratory report, submit it here.Part 1Virtual laboratory: Gene ControlAs you learned in Chapter 5, a number of chemical reactions can influence whether a gene is switched on or off. For example, genes may be switched on at times when proteins are depleted and new proteins are needed. These activities occur in many locations throughout the body. Gene expression is regulated by activator and repressor proteins present in cells. The field of epigenetics involves heritable changes in gene expression that doesn't involve a change in DNA sequence. Oftentimes these signals come from the environment, from things like diet or stress. The most well studied epigenetic regulator is methylation of the genome, which "turns off" gene expression. In this lab you will act as an epigenetic signal to see what happens to gene expression and protein synthesis.This laboratory experiment will allow you to see how signals you apply will result in a change in gene expression in a cell. This laboratory is from the University of Utah's Genetic Science Learning Center. Enter the laboratory here_(new window). In the laboratory you will see a diagram with three rectangular windows along the left (GFP gene, GFP mRNA, and Green Fluorescent Protein). In the upper right you have a dial, a sound on/off button and a label button. Below this area is the cell. Procedure: Click on i in each of the windows to read a description of the lab materials (GFP gene, histone, methyl tag, acetyl tag, GFP mRNA, and Green Fluorescent Protein). Turn the dial so that the smaller circle is in the 7 oclock position. Turn on the labels (the green indicator will light up) and note the sequence that will occur.Begin the experiment by turning the dial clockwise. Observe what happens as you increase the signal. Answer the following questions in your lab report. Your report will be entered in this link.Lab Questions (Note: a separate answer sheet is required for submission (attached). Download it, save it as a Rich text file rtf, add your answers to the questions, save and submit it in the designated area). When you move the dial from the 7 o'clock position to the 9 oclock position, describe what happens in the GFP gene window.At this 9 oclock position, what effect does this increased signal have on transcription, and protein synthesis as seen in the other two windows?As compared to the first position (7 oclock), what happens to the methyl tags as you increase the signal to the 12 oclock position?Describe the appearance of the genome when:a gene is activegene is inactiveWhat action allows the acetyl tags to become visible? Part 2Scientific MethodRead the next item in our virtual lab: Could you really do this?_(new window)Lab Question6. Based upon what you have read,Generate a hypothesis for this experimentIndicate the dependent and independent variables Lab I lab report rev.docx Lab I lab report rev.docx August 8 2018, 6:49 PM
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